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22cccС˵,003uuС˵,jizlee

g[Δ7477 ڣ2020-10-27 11:52:27

ժҪ

(1)

1ELISAzyõĘӱMвȡͱ棿

ጣ󲿷ELISA zyѪѪ{ӱѪӱɰҎ22cccС˵,003uuС˵,jizleeɼ]Ѫtܽrጷų^øԵ|HRP ӛELISA yУѪӱܕӷخ@ɫѪӱrrzyм@ȾwпܺЃԴHRPҲaԷҼf5 ȜyѪӱɷ28棬ӱڱбrg^LѪIgG ۺϣ؄eʹgӷԇı׼^ҼܜyĘӱ裭20±棬YѪڽᣬ|ֲsֲֻKaݡյѪӱxĻ^Vٙzy̓ڕʹwЧr䣬ԜywѪӱ豣Ιzyb档ѪԲɼr͑]oҲɼmȫĘӱwSԭĎ֔_ɼԣhMÿѪǸؿ

(2)

2ELISAzyr22cccС˵,003uuС˵,jizleeCЧϴ죿

ጣϴnjخY춹Ŀԭwc^ķخɷݷx_ԱCELISAyخԡˣϴ匦ELISAyfǘOPIҼh

ҽhʹȫԄϴCϴ죬ֹϴɽ@Ⱦ

ڌϴCMоSoBʹǰzПo¿׬FҺ]Һ߰22cccС˵,003uuС˵,jizlee\ǷҼҪÿ׵Ě^2ul]Һÿ״250ul磬ͬrOҼĽݕrg60/飬ϴ5

(3)

3ELISAɫІβLpLą^e

1Йnϵø˃x϶ͬrІβLpLɫܣ^ĆβLyx񌦽Kֹ@SɫҺwյIJL450nmMбɫyõ׵AֵдAֵ˱ҺwɫֵW1ͷخֵW2خֵҼֵ׼׵ָyκۡ҉mKֵtW1=A-W2҂ɫrOÿհ{㣬ǰخֵMp䌍Kÿ׵ķخֵҼӵģʆβLyҼ̶ȵIJ_ԣҲfհ׿λòͬпܵõֵֻͬڿɽܹȡ

2Lpɫtø˃xвL450nmͷвL630nm¸yҼΣвLȜyֵخ@ɫcָyκۡ҉mKµ֮ͣM1вLœy,˕rخ@ɫֵ㣬yõȼKֵ(M2)ø˃xoĔֵвLµֵcdzвLµֵIJA=M1-M2, ʶڜyɫrʹpLɫ

3xpLɫrOհ׿{㣬Oհ׿{ɫпܕFȞؓ22cccС˵,003uuС˵,jizleeĬF

(4)

4ELISAzyԇжƫ͵̎

   
1 Œ򞣬Kڌ]ԇеĜضƽ⡢ĕrg@ɫĕrgӰصĿƣ
2 z|ƷǷз̓ڵr|ƷǷȲ|ƷǷ28ã
4 u|ƷĿɿԣ
5 ӱ춸NӰ푶FԣHϞ22cccС˵,003uuС˵,jizleeӱ
6 춘ӱ^ͣSˮƽӡ@֘ӱ̎푪أЗlÑMд_C򞣻
7 ]z錍õăxOǷУʣ
8 ]z錍õİ塢øԇ̖Ƿ̖ͬͬԇûá

(5)

5ELISAaƷzyrFƫ߻ߡ塱FҪԭ

ϴԭ

1SʹõϴҺ䷽ԇėlƵģò׵ϴҺ_ϴYҪ22cccС˵,003uuС˵,jizleeɱ^ߣFF󡣱˾ϴϵyh˾ԇлá

2ϴҺˮԓrsˮȥxˮʹԁˮˮк^Ca2+Mg2+@ЩxӕռñԄӰϴЧͬrˮҼЩ΢ጷŵăԴ|Ɏ֔_F^ߵķخԷ

3ϴrCϴCÿ]ҺҺʹãtӰϴЧҼ]ҺҪ300350l/ף⽻@ȾҺ2lϴ5

(6)

@ɫϵyԭ

1BҺ΢׃ɫɱƫߡ

2ʹõĘ^ӘӲϴҲ֔_ʹõABҺlخ

ӱԭ

1ʹѪӱtܽrጷų^øԵ|ӷخ@ɫѪӱ^22cccС˵,003uuС˵,jizleefм@ȾwпܺЃԴHRPҲӷخ@ɫ

2ʹ˿ȫĘӱwSԭĎ֔_ɷخ@ɫhMÿѪǸؿӱ

e P֪R

ԓYӍPI~飺

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